Next Generation Sequencing

Next Generation Sequencing (NGS) is a revolutionary technology which is based on massive-parallel sequencing of short DNA-fragments. Millions of DNA fragments are produced in parallel allowing for ultra deep sequencing, which following sophisticated bioinformatic analyses can be realigned to a reference sequence. The main application is genome or exome sequencing in humans and animal or plant models. Numerous methodological developments have been made allowing also other applications such as transcriptome and methylome profiling or chromatin-immunoprecipation sequencing, all of which were previously performed using microarray based experimental designs.

This Core Units was established within IZKF in 2007 under the denomination of Genomics Unit (Z3) operating an Affymetrix microarray platform. At the end of 2010 a first NGS platform was acquired. Following the rapid technological developments, at the end of 2013 already the third generation of instrumentation was put into operation. Thus the Core Unit has state-of-the-art instrumentation and methodology as well as a dedicated bioinformatic support providing all major applications to its users. Following the initial IZKF funding, the Core Unit is funded since late 2012 by the Faculty of Medicine in collaboration with the Biology Department of the Natural Science Faculty.